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KMID : 0364820140500040345
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Korean Journal of Microbiology
2014 Volume.50 No. 4 p.345 ~ p.350
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Viability of Probiotics in Feed under High Temperature Conditions and Their Growth Inhibitory Effect on Contaminant Microbes
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Kim Gyeom-Heon
Lee Kwon-Jung
Lee Ah-Ran
Jang In-Hwan
Song In-Geun
Kim Dong-Woon
Kim Soo-Ki
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Abstract
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The aim of this study was to investigate the effect of high temperature on the viability of probiotic organisms
(Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) mixed with animal feed under controlled
conditions by simulating a farm feed bin in the summer. Following inoculation of probiotics into the feed, the pH
and probiotic viability were monitored during an 8-day incubation at room temperature. Sterile and non-sterile feeds
displayed different patterns of pH changes, with increased pH in non-sterile feed at 2 days, but a pattern of
decreasing pH at 4 days. The viabilities of S. cerevisiae and B. subtilis after mono/co-inoculation were maintained
without substantial changes during the incubation, whereas L. plantarum viability tended to decline. In both
non-sterile and sterile feeds, the probiotics were maintained or grew without any antagonistic effects. Probiotic
viability was also tested upon a shift to high temperature (60℃). There was no distinct change in pH between sterile
and non-sterile feeds after the temperature shift. L. plantarum and S. cerevisiae could not survive at the high
temperature, whereas B. subtilis displayed normal growth, and it inhibited the growth of contaminant microbes.
Fungal growth was not observed in non-sterile feed 2 days after supplementation with B. subtilis. Therefore, heat
resistant B. subtilis could be safely used in feed bins to inhibit microbial contamination, even at high temperatures.
The prevention of elevated temperature in feed bins is necessary for the utilization of L. plantarum and S. cerevisiae
during the summer season.
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KEYWORD
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feed bin, high temperature, probiotics, viability
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